Brunel Microscopes Ltd

Registered in U.K. (England) No: 2060047

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As the first company to introduce LEDs into fluorescence microscopy, CoolLED has developed a comprehensive understanding of the complexities of configuring and selecting filter sets for samples which have multiple stains. These ’multi-band’ filters must have a corresponding absorption and emission band for each stain and the bands must be kept spectrally separate to ensure there is no overlap.

CoolLED’s innovation comes from recognizing that all the stains used in multi-band work can have their absorption bands divided into four separate groups across the spectrum due to the restricted bandwidth available. This has allowed the development of a patent-pending, wavelength-grouping concept which makes it possible to deliver more power in an efficient four channel system.

With its unique 16 selectable LED wavelengths, the pE-4000 ensures optimum compatibility with all single and multi-band filter sets. The flexibility and extensive functionality of the pE-4000 broadens the range of illumination options in core facilities.

The compact pE-4000 Light Source houses all the LEDs, their combining optics, thermal management and control electronics. A dual mode control pod gives manual control in either White or Advanced modes. Light is delivered to the microscope by either a single liquid light guide (LLG) or fiber. LLGs can be used in conjunction with CoolLED’s pE-Universal Collimator which can accept a microscope adaptor from CoolLED’s extensive range.

The pE-4000 offers:-

• Broadest LED spectrum available: 365-770nm – match LED peaks to existing microscope filters
• Four channel wavelength-grouping design matches with all commercially available dual, triple, and quad filters
• Individual control of selected LED wavelengths – less background, no filter wheel or shutters
• Integrated with many imaging software packages - can be incorporated into all experimental parameters
• Instant on/off – no shutters required, no warm up or cool down
• Stable & repeatable – reliable and consistent results
• Wide range of Microscope Adaptors – fits most microscopes
• Precise intensity control in 1% steps (0-100%) – no ND filters required
• Excellent uniformity over field of view – fixed and stable, no alignment necessary
• Long lifetime – expected to exceed 25,000 hours of operating time

IDEAL FOR MULTI-USER AND CORE FACILITIES

• Pre-sets allowing lab manager to match white spectrum to existing filter cubes
• Higher contrast images from matched-white spectrum
• Simple to fit, simple to use
• No training required

IDEAL FOR ADVANCED RESEARCH

• Individual LED wavelength selection
• Rapid switching between LED wavelengths enables capture of high speed events
• TTL & USB interfaces with imaging packages
• Excitation filters can be fitted in optical path for controlled switching with no moving parts
• Analogue inputs for dynamic intensity control
• Optical feedback for applications requiring higher stability
• Internal function generator for electrophysiology and optogenetics applications

Price: £7595 + vat

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