SP105 Fluorescence

Brunel Microscopes Ltd

Registered in U.K. (England) No: 2060047

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Excellent brightfield transmission and incident fluorescence microscope

Brunel SP105F Inverted Fluorescence Microscope Accessories

The Brunel SP105F has a range of practical accessories available in our online shop.

Inverted & Fluorescence Microscopes

Brunel SP300F

Brunel SP99F

CoolLED Light Units

Accessories

The Brunel SP-105F is our ‘flag ship’ inverted fluorescence microscope that produces a superior microscopic image, providing an outstanding performance and great value for money. The fluorescence cubes use superior quality filters that include bandpass barrier filters that reduce colour bleeding significantly. The SP105F represents a new generation of infinity colour corrected optical systems with widefield high eyepoint eyepieces and plan achromatic objectives, The microscope body has an ergonomic design and the optical system has a notable long working distance.

Three series of objectives are available with the SP105F and the exact configuration can be chosen to suit the application.

LPLAN - long working distance plan colour corrected objectives specifically designed for living cell cultures that also have a good performance with fluorescence observation.

LPAN PH - long working distance infinity phase contrast objectives. In addition to good bright field observation these objectives have been developed to show much improved relief, image contrast and resolution.

LPLAN-FLUOR - these are infinity plan semi-apochromatic fluorescence objectives with a high numerical aperature and excellent aberration correction. The objectives have wideband multicoating technology

LPLAN-FLPH - infinity plan semi-apochromatic phase contrast fluorescence objectives that give excellent results with bright field, phase contrast and fluorescence.

Focussing SLR camera adapter. SP105F-01	£165.00	x16 eyepieces (pair) SP105F-06	£90.00
Hg 100watt lamp SP105F-02	£146.67	Replacement security screen SP105F-07	£20.83
6v 30watt halogen lamp SP105F-03	£9.17	Blank filter cubes SP105F-08	£138.33
x10 measuring eyepiece SP105F-04	£55.00	V (violet) filter cube SP105F-09	£326.67
Stage micrometer SP105F-05	£29.17	CCTV adapter SP105F-10	£47.50

SP105F Hg lamp unit

SP105F condenser system

SP105F Hg control box

Brunel SP105F

Series	Magnification	N.A.	W.D. mm	Coverglass thickness
L PLAN Series Objectives	Lplan x4	0.1	22	1.2
	Lplan x10	0.25	7.94	1.2
	Lplan phase x10	0.25	7.94	1.2
	Lplan x20	0.4	7.66	1.2
	Lplan phase x20	0.4	7.66	1.2
	Lplan x40	0.6	3.71	1.2
	Lplan phase x40	0.6	3.71	1.2
L PLAN semi-apochromatic series objectives	Lplan fluor x2	0.06	25.05	-----
	Lplan fluor x4	0.13	18.52	1.2
	Lplan fluor x10	0.3	7.11	1.2
	Lplan x20	0.45	5.91	1.2
	Lplan fluorphase x20	0.45	5.91	1.2
	Lplan fluor x40	0.65	1.61	1.2
	Lplan fluorphase x40	0.65	1.61	1.2
	Lplan fluor x60	0.75	1.04	1.2

The mechanical stage has a movement range of 120 x78mm and a range of pre shaped camps are available to fit the more common cell culture vessels and plates. There is also an insert that will accommodate a standard 3 x “ microscope slide. The entire mechanical stage assembly can be removed easily by releasing two thumb screws on the underside of the stage.

The transmitted illumination is 6v 30watt and the condenser housing has a slot for the insertion of the appropriate phase rings for phase contrast.

The fluorescence filter blocks are housed in a switching mechanism. The SP105F has three fluorescence channels and a single bright field channel. The filters supplied as standard have the characteristic shown on the graphs towards the end of this web page. Custom cubes can be supplied at additional cost. The SP105F has as standard a mercury lamp power supply and control system with an inbuilt protection system. If the side door to the lamp housing is not properly closed, the power circuit to the DC mercury lamp will be automatically cut avoiding the escape of UV light. The digital power control system has a current and total working time display with a reset button and safety trigger.

The SP105F can also be supplied or retro fitted with CoolLED units pE-100, pE-300 and pE-7000.

The microscope body has a coaxial focus system with tension adjustment and excellent fine adjustment precision of 0.002mm

Price: SP105F with mercury lamp £8525.00 + vat

SP105F with CoolLED pE-300 £13450.00 + vat

Cell culture of 293T cells shown under phase contrast and showing transfection with Green Fluorescence Protein (GFP)

SP105F Gemel trinocular head

SP105F with CoolLED pE-300

We can supply a number of camera units ideally suited to fluorescence work. The choice depends on budget and application. Black and white CCD chip cameras provide the best resolution and we have both standard and cooled units.

The images above were taken with a non cooled Sony CCD black and white chip unit and the colours restored and images stacked using software